Standard Operating Procedure (SOP) for HPLC Calibration

Objective To outline the procedure for the calibration of High-Performance Liquid Chromatography (HPLC) equipment to ensure accurate flow rate, injector precision, detector linearity, and column oven temperature, meeting specified criteria. Scope This procedure applies to the calibration of HPLC systems used in analytical laboratories, specifically for: Accuracy of Flow Rate: 0.5 mL/min and 5 mL/min. Injector Precision: 20 μL injection […]